Concerning the problems of low recognition efficiency and high misreading rate on the occasions of many tags which move fast, an anti-collision algorithm based on packet-first then handling-second was proposed. The algorithm reduced the misreading rate by to grouping the tags according to the order of arriving, it could adaptively adjust frame length based on slot situation to improve the search efficiency, and use jumping dynamic searching algorithm to deal with conflict slots, which could reduce the number of search for readers and the transmission of system. Matlab simulation results show that the algorithm’s communication complexity is lower than other commonly used algorithms, and throughput can achieve 0.59~0.6. The larger the number of tags is the more obvious superiority of the algorithm is.